Real-Time Multiplex PCR Assay and Melting Curve Analysis for Identifying Diarrheagenic Escherichia coli
نویسندگان
چکیده
منابع مشابه
Detection of diarrheagenic Escherichia coli by use of melting-curve analysis and real-time multiplex PCR.
Diarrheagenic Escherichia coli strains are important causes of diarrhea in children from the developing world and are now being recognized as emerging enteropathogens in the developed world. Current methods of detection are too expensive and labor-intensive for routine detection of these organisms to be practical. We developed a real-time fluorescence-based multiplex PCR for the detection of al...
متن کاملMultiplex PCR assay for identification of human diarrheagenic Escherichia coli.
A multiplex PCR assay for the identification of human diarrheagenic Escherichia coli was developed. The targets selected for each category were eae for enteropathogenic E. coli, stx for Shiga toxin-producing E. coli, elt and est for enterotoxigenic E. coli, ipaH for enteroinvasive E. coli, and aggR for enteroaggregative E. coli. This assay allowed the categorization of a diarrheagenic E. coli s...
متن کاملMultiplex real-time PCR assay and melting curve analysis for identifying Mycobacterium tuberculosis complex and nontuberculous mycobacteria.
A multiplex real-time PCR assay and melting curve analysis for identifying 23 mycobacterial species was developed and evaluated using 77 reference strains and 369 clinical isolates. Concordant results were obtained for all 189 (100%) isolates of the Mycobacterium tuberculosis complex and 169 (93.9%) isolates of nontuberculous mycobacteria. Our results showed that this multiplex real-time PCR as...
متن کاملMultiplex real-time PCR melting curve assay to detect drug-resistant mutations of Mycobacterium tuberculosis.
Early diagnosis of drug-resistant Mycobacterium tuberculosis is urgently needed to optimize treatment regimens and to prevent the transmission of resistant strains. Real-time PCR assays have been developed to detect drug resistance rapidly, but none of them have been widely applied due to their complexity, high cost, or requirement for advanced instruments. In this study, we developed a real-ti...
متن کاملA multiplex real-time PCR melting curve assay to detect drug resistant mutations of Mycobacterium tuberculosis
1 Early diagnosis of drug resistant Mycobacterium tuberculosis is urgently needed to 2 optimize treatment regimens and to prevent the transmission of resistant strains. Real3 time PCR assays have been developed to detect drug resistance rapidly, but none of them 4 have been widely applied due to their complexity, high cost, or requirement for advanced 5 instruments. In this study, we developed ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2013
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.02478-12